Sample Dilution Technology
Discover AnySource® – the advanced sample dilution technology from Chimera Biotec. Developed in parallel with the ultra-sensitive Imperacer® platform, AnySource® optimizes immunoassays through high dilution tolerance. Benefit from minimal sample consumption and maximum sensitivity for preclinical and clinical studies
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20 years of experience
Ultra-sensitive bioanalytics – GLP/GCP compliant!
Experience the future of bioanalytics with chimera biotec! Our GLP/GCP-compliant services offer you the highest precision and reliability. With our award-winning Imperacer® technology, we achieve ultra-sensitive detection that goes far beyond conventional methods. Trust in over 20 years of experience and let us master your bioanalytical challenges
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Maximize your research results with Chimera Biotec’s ultra-sensitive assays – without expensive investments!
Chimera offers customized ultra-sensitive ligand binding assays that go beyond the limits of classical immunoassays without the need to invest in expensive laboratory equipment. Rely on our long-standing expertise and outsourcing solutions for challenging case studies where conventional ELISA quantification is not sufficient.
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Precise analyses with minimal sample volumes
Revolutionizing Multi-Analyte Testing: Polyplex and Imperacer® Technology for Ultra-Sensitive, Simultaneous Measurements
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Precise analyses with minimal sample volumes
Microsampling enables precise bioanalytical tests with minimal sample volumes, ideal for sensitive and rare matrices.
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Expertise | AnySource® Sample Dilution Technology
Anysource |
Sample dilution technology
Rare matrices and multianalytical support
Optimizing immunoassays with advanced sample dilution technology
Sample dilution is a powerful tool in the optimization of immunoassays.
Developed in parallel to its ultra-sensitive Imperacer® platform, Chimera advanced AnySource®, a sample dilution technology to take full advantage of Imperacer’s® strong tolerance for sample dilution.
The minimum required dilution (MRD) effect – optimal immunoassay performance in a diluted sample – is well known regarding immunoassays in general. Ultra-sensitive platforms providing a broad assay range such as Imperacer® or Simoa®, however, allow for potential greater sample dilution to further minimize matrix effects while maintaining sensitivity.
Besides, this gives the option to support rare matrix or microsampling studies with extremely reduced sample consumption at sensitivities still way better than conventional immunoassays. In this regard, Imperacer® might be favorable due to technical factors which lead to less required sample per well.
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Optimized sample dilution buffers and procedures
Tailored buffer pptimization with AnySource® for superior assay performance
In a design of experiment (DOE) approach, buffer conditions are optimized by running dilution experiments in ten standard AnySource® diluents.
Each standard buffer represents a family of AnySource® sample dilution buffers (SDB). Based on the experimental outcome, a customized AnySource® SDB buffer for the particular assay is identified from our library or developed according to the buffer attributes which performed best.
AnySource® is also employed for fine tuning or converting an assay, e.g. from being ultra-sensitive to microsampling support with minimal sample consumption.
The handling and storage of low volumes of biological matrices is challenging. Pipetting of volumes below 10 µl can lead to higher variability, strong evaporation effects, and sample loss in frozen storage. Even though we employ highly trained technicians at Chimera who are well equipped to handle sample volumes down to 0.5 µl, we acknowledge the basic requirements and limitations in preclinical and clinical trials.
To analyze any biological matrix and the desired target(s), a mutual AnySource® storage buffer is developed. This diluent is designed to be compatible with stability requirements for long-term frozen storage of the analyte in the diluted specimen, as well as with downstream assay requirements. Pipetting volumes can be adjusted regarding available sample volume and minimal pipetting volume that can be adjusted to study requirements.
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How it works
Maximizing efficiency and specificity with precise sample management
Taking backup volume for potential re-testing into account, dead volume is avoided and only the sample material needed for the bioanalytical assays is shipped to Chimera.
Residual material can be used for additional procedures or be stored with the sponsor or central lab.
Upon receipt of the pre-diluted sample at Chimera’s GLP/GCP compliant analytical facility, working aliquots are prepared. Once more, the amounts pipetted are typically in manageable volumes of more than 10 µl.
Each working aliquot can be diluted a second time in assay specific AnySource® sample dilution buffer (SDB), converting the buffer conditions from storage prerequisite to optimal analyte binding for the particular antibody pair used in the actual assay.
Finally, quantification data are generated from parallel assays run in duplicate wells. In contrast to typical multiplexing immunoassays, this Polyplexing® approach ensures the highest possible, antibody intrinsic specificity as antibody cross talk is completely avoided
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Depending on sensitivity requirements, typically 10 analytes (e.g., drug (PK) plus 9 biomarkers) can be tested from a 25 µl sample. Taking backup volume into account, 50 µl of neat sample volume is sufficient for testing 10 analytes in parallel, duplicate well assays with single digit pg/ml sensitivities each.
Please read further in this rare matrix case study from ophthalmology drug development support.
Locations of the laboratories
Location advantages
Bremen
Our laboratory location in Bremen offers ideal logistical conditions for efficient sample delivery. Thanks to the excellent transport connections, your samples will reach us quickly and reliably. We use our strategically located site to map our fast and smooth logistics processes.
Dortmund
Our laboratory location in Dortmund benefits from the dynamic innovative power of the city, which is known as an important center for technology and research. Together with our laboratory in Bremen, we offer you a high level of redundancy and additional security for your projects thanks to two locations. Take advantage of our first-class infrastructure and secure reliable and continuous support for your biotechnological requirements!
Enhanced efficiency and accuracy
The ability to fine-tune buffer conditions not only improves assay performance but also reduces the risk of cross-reactivity and other common issues associated with multiplexing. By employing the Polyplexing® approach, AnySource® ensures that each analyte is detected under optimal conditions, leading to more reliable and reproducible results. This level of precision is essential for studies that require high sensitivity and specificity, such as those involving oncology, neurology, and other complex disease areas.
Conclusion
OurAnySource® sample dilution technology is more than just a tool for reducing background noise and maximizing sample use. Its true strength lies in its flexibility and precision, making it the ideal choice for researchers facing the challenges of modern bioanalytics. By providing tailored solutions for diverse sample types and conditions, AnySource® empowers scientists to push the boundaries of what is possible in ultra-sensitive bioanalysis. Trust Chimera to deliver the innovative solutions you need to elevate your research to new heights.
Unleashing the full potential of AnySource®: Flexibility and precision in sample dilution
Tailored solutions for diverse sample types and conditions
In the dynamic field of bioanalytics, the ability to adapt to various sample types and conditions is crucial. This is where the AnySource® sample dilution technology from chimera biotec truly shines. Beyond its well-documented benefits, AnySource® offers unparalleled flexibility, making it an indispensable tool for researchers working with rare or hard-to-access samples.
Flexibility in sample dilution
One of the standout features of AnySource® is its ability to customize buffer conditions to meet the specific needs of each experiment. This flexibility ensures that even the most challenging samples can be processed with optimal efficiency and accuracy. Whether dealing with rare matrices or microsampling, AnySource® allows for precise adjustments that enhance the overall performance of bioanalytical assays.
Optimized for ultra-sensitive detection
AnySource® is designed to work seamlessly with ultra-sensitive platforms like Imperacer®. By leveraging the strong tolerance for sample dilution inherent in these platforms, AnySource® enables researchers to achieve exceptional sensitivity and specificity. This is particularly beneficial when working with limited sample volumes, as it allows for the detection and quantification of multiple targets from a single source without compromising on data quality.
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Expertise | Ultra Sensitive Ligand-Binding Assay Capabilities
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